lambda protein phosphatase kit  (New England Biolabs)

Journal: bioRxiv

Article Title: Rare bioactive tau oligomers from Alzheimer brain support both templated misfolding and fibril formation

doi: 10.1101/2025.09.24.678227

Figure Lengend Snippet: A) Schematic representation of HMW tau dephosphorylation by the Lambda Protein Phosphatase (LPP). B) Seeding activity assay using FRET reporter HEK cell; the integrated FRET intensity was quantified by flow cytometry. Data represents mean ± S.D. of three individual experiments performed in triplicate with HMW Tau isolated from the AD case 1971. Results were analyzed using the unpaired student t-test, ** for p < 0.005. C), D) Dot blot quantification of HMW tau, originating from the same case as panel B, treated or not with Lambda PP and probed with the anti-phospho tau AT8 or the total tau antibody D5D8N.

Article Snippet: HMW tau was subjected to dephosphorylation using Lambda Protein Phosphatase (LPP) kit (New England Biolabs (NEB), #P0753S).

Techniques: De-Phosphorylation Assay, Activity Assay, Flow Cytometry, Isolation, Dot Blot

Journal: Science Advances

Article Title: Cyclin F–EXO1 axis controls cell cycle–dependent execution of double-strand break repair

doi: 10.1126/sciadv.ado0636

Figure Lengend Snippet: Reagents, antibodies, and cell lines used in the study. IgG, immunoglobulin G; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HRP, horseradish peroxidase.

Article Snippet: In vitro dephosphorylation was performed using λPP kit (New England Biolabs, P0753S).

Techniques: Agarose Gel Electrophoresis, CRISPR, Recombinant, Conjugation Assay, DNA Extraction, Membrane, Protease Inhibitor, Lysis, Expressing, Plasmid Preparation